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Custom stitching jobs of large terabyte-sized datasets using Stitchy
Light Sheet Stitching is the third step of our modular custom service offerings, delivering complete 3D datasets of clients’ native light sheet files.
Handling large terabyte-sized datasets can be challenging with standard stitching pipelines. To streamline the light sheet stitching workflow, our internal team has developed Stitchy.
Stitchy converts data from your native microscope file type to your preferred format, ensuring seamless integration with downstream quantification applications.
Learn more about light sheet stitching on our science page.
Simply send us your raw light sheet files, and we’ll return the output in your preferred format.
Supported File Types:
Supported Output Formats:
We also support multi-view and dynamic focus data in the formats listed above.
If your file type is not on the supported list, let’s collaborate. We are continually striving to increase compatibility and would like to work with you. Please schedule a 15-minute call with our team or reach out to us at info@translucence.bio.
Browse actionable insights generated by the
Light Sheet Image Stitching
service
Browse through images of some of the light sheet images and videos that have been generated by our Custom Light Sheet Stitching services
Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.
This spinal cord sample was prepared using Translucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
This kidney sample was stained with Smooth Muscle Actin (SMA) and prepared using theTranslucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
Tyrosine Hydroxylase (TH) staining of a whole mouse brain visualizes the distribution of dopamine, norepinephrine, and epinephrine-containing (catecholamine) neurons and endocrine cells. TH is a key enzyme involved in neurotransmitter production. Immunohistochemical detection of TH expression is one tool for visualizing and quantifying damage and loss of dopaminergic neurons, involved in disorders including Parkinson’s disease. Sample imaging performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.
Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.
This spinal cord sample was prepared using Translucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
This kidney sample was stained with Smooth Muscle Actin (SMA) and prepared using theTranslucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
Tyrosine Hydroxylase (TH) staining of a whole mouse brain visualizes the distribution of dopamine, norepinephrine, and epinephrine-containing (catecholamine) neurons and endocrine cells. TH is a key enzyme involved in neurotransmitter production. Immunohistochemical detection of TH expression is one tool for visualizing and quantifying damage and loss of dopaminergic neurons, involved in disorders including Parkinson’s disease. Sample imaging performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.
Light Sheet Stitching delivers complete 3D datasets of your native light sheet files.
Interested in performing stitching in-house? Stitchy™ is a light sheet image stitching tool that streamlines your light sheet stitching workflow– performing 2.5-5.5x faster than leading commercial software solutions.
Learn more about Stitchy™ →
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