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High-resolution, three-dimensional imaging of large intact samples with unprecedented speed and resolution
3D Tissue Imaging is the second step of our modular custom service offerings, delivering precise and robust 3D datasets of cleared tissues. We accommodate a wide variety of samples and tailor the resolution to meet the specific needs of each client.
Light sheet Fluorescence Microscopy (LSFM) utilizes a thin planar sheet of excitatory light to selectively illuminate a thin optical section of the sample at a time. This approach minimizes out-of-focus light and reduces photobleaching compared to traditional epifluorescent microscopy. This allows for high-resolution imaging deep within optically cleared samples.
Depending on the experimental requirements, researchers can prioritize high spatial resolution for detailed structural analysis, or optimize for faster imaging speeds to capture targets when high spatial resolution is not required.
Translucence Biosystems offers custom imaging services using the ZEISS Lightsheet Z.1 utilizing the Mesoscale Imaging System, designed to meet the demands of large, cleared tissue samples. This enables the acquisition of high-resolution, three-dimensional images of large intact samples. We offer custom resolution selection, tailored to address your specific biological questions.
Custom 3D tissue imaging is a versatile service that provides detailed, high-resolution insights. This makes it suitable for a multitude of research applications across various fields.
Learn more about tissue imaging on our science page.
Browse actionable insights generated by the
3D Tissue Imaging
service
Browse through images of some of the cleared tissues that have been generated by our Custom 3D Imaging Services
Embryonic β-Tubulin staining showcases the intricate structure of a mouse embryo. This footage is brought to light using Light Sheet Fluorescence Microscopy (LSFM) techniques, imaged on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™️. Sample preparation performed using our NIH-funded Universal Tissue Clearing Kit.
Whole mouse liver stained with Smooth Muscle Actin (SMA) in Cyan and Cytokeratin 7 (CK7) in green. Tissue clearing and staining for SMA and CK7 enables the use of Light Sheet Fluorescence Microscopy (LSFM) to visualize vasculature and bile duct networks – providing comprehensive insights into the 3D morphology of both targets.
Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.
This spinal cord sample was prepared using Translucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
This kidney sample was stained with Smooth Muscle Actin (SMA) and prepared using theTranslucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
Volume quantification tool used on in-house 3D Tyrosine Hydroxylase staining. Our newly developed volume quantification tool does not segment individual objects, but measures the total fluorescence in each brain region. Because TH staining is very different in different brain regions, segmentation is problematic, so we used this new tool to calculate TH integrated intensity across hundreds of brain regions.
Visualization of liver vasculature using Smooth Muscle Actin (SMA) in the whole mouse liver. Tissue clearing and staining for SMA enables the use of lightsheet imaging to visualize the vascular networks in the liver. This approach provides robust insights into vascular networks, often implicated in liver diseases. Tissue clearing provides more comprehensive insights compared to traditional 2D slice immunohistochemistry (IHC) methods. Sample preparation was performed using the Translucence Universal Tissue Clearing Kit. This NIH-funded kit is suitable for clearing various tissue types, including mouse brain, lung, liver, spinal cord, and more. Imaging was performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.
This image shows a mouse brain stained for smooth muscle cells surrounding arteries using tissue-clearing and immunolabeling methods. This helps scientists visualize the blood-brain barrier, a critical barricade that limits the entry of therapeutics into the brain. This image was featured in the 2024 BRAIN Initiative Calendar.
Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.
3D Tissue Imaging delivers robust 3D datasets of cleared tissues. We accommodate a wide variety of samples and tailor the resolution to meet the specific needs of each client.
Interested in imaging large intact tissues at your facility? The Mesoscale Imaging System expands the capabilities of your ZEISS Lightsheet Z.1 microscope, allowing you to capture high-resolution, three-dimensional images of larger intact samples with unprecedented speed and resolution.
Schedule a 15-minute call with a member of our team where we’ll provide recommendations tailored to your use case.
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